High fat diet-induced modifications in membrane lipid and mitochondrial-membrane protein signatures precede the development of hepatic insulin resistance in mice

In this survey we effectively combined transcriptomics, proteomics and targeted-metabolomics to analyse the temporal relationship of alterations in liver preceding and accompanying the development of HFD-mediated hepatic insulin resistance. To assess HFD-mediated alterations in physiological parameters, insulin sensitivity, and molecular adaptations in liver male C3HeB/FeJ mice treated with a high-fat diet (HFD) for 7, 14, or 21 days and compared to age- matched controls fed low-fat diet (LFD).

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Kahle M, Schäfer A, Seelig A, Schultheiß J, Wu M, Aichler M, Leonhardt J, Rathkolb B, Rozman J, Sarioglu H, Hauck SM, Ueffing M, Wolf E, Kastenmueller G, Adamski J, Walch A, Hrabé de Angelis M, Neschen S