Probing off-target effect of LMNA siRNA and pro-siRNA in HeLa-d1EGFP cell line

We have develped a novel method of making siRNAs (named pro-siRNA for prokaryotic siRNA). To evaluate off-targeting of pro-siRNA, we compared mRNA expression profile of HeLa-d1EGFP cells transfected with 4 nM LMNA siRNAs and pro-siRNAs by microarray.

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Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J