Identification of genes responsive to mild hyperthermia in human skin normal fibroblast Hs68 cells

Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41C for 30 min (mild hyperthermia) on the gene expression in Hs68 human skin normal fibroblast cells were investigated using an Affymetrix GeneChip system. The cells were treated with mild hyperthermia, followed by incubation for 0, 1, or 3 h at 37C. No cell death was observed in the mild hyperthermia-treated cells. On the other hand, many genes that were differentially expressed by a factor 1.5 or greater were identified in the cells treated with the mild hyperthermia.

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Tabuchi Y, Furusawa Y, Kariya A, Wada S, Ohtsuka K, Kondo T