Microarray analysis of normal and abnormal chick myocardial development

The goal of this study was to determine developmental differences in gene expression between left and right ventricle, and to assess the differential effect of altered hemodynamic loading on left and right ventricle. Chick ventricles from different developmental stages were isolated for assessment of normal developmental profiles. Conotruncal banding or partial ligation of the left atrial appendage was performed in ovo at embryonic day 4 and ventricles were isolated at embryonic day 5 (banding) or 8 (ligation) for assessment of altered loading effects.

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Krejčí E, Pesevski Z, DeAlmeida AC, Mrug M, Fresco VM, Argraves WS, Barth JL, Cui X, Sedmera D