Gene expression profiling in BCR/ABL expressing LSCs and BCR/ABL expressing Alox5-/-LSCs

We previously demonstrated that Alox5deficiency impairs the function of LSCs and prevents the initiation of BCR-ABL-induced CML. To identify the pathways in whichAlox5gene regulates function of LSCs, we performed a comparative DNA microarray analysis using total RNA isolated from non-BCR-ABL-expressing Lin-Sca-1+c-Kit+, BCR-ABL-expressing wild type LSCs and BCR-ABL-expressing Alox5-/- LSCs. The result was validated by quantitative real-time PCR analysis of non-BCR-ABL-expressing Lin-Sca-1+c-Kit+, BCR-ABL-expressing wild type LSCs and BCR-ABL-expressing Alox5-/- LSCs.

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Chen Y, Sullivan C, Peng C, Shan Y, Hu Y, Li D, Li S