Expression profiling of MCF10A cells in 2D and 3D culture

In these microarray experiments, we characterize the gene expression of mammary epithelial cells (MCF10A cells) grown in either a traditional monolayer cell culture setting (2D) or on Matrigel, which induces single MCF10A cells to form organized acinar structures (3D). Morphogenesis of mammary epithelial cells into organized acinar structures in vitro is accompanied by widespread changes in gene expression patterns, including a substantial decrease in expression of Myc.

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Simpson DR, Yu M, Zheng S, Zhao Z, Muthuswamy SK, Tansey WP