RNA profiles of embryonic stem cells generated from in vitro produced or in vivo derived rhesus preimplantation embryos

It is often overlooked that human ESCs are generated from in vitro cultured, often surplus/discard, embryos considered unsuitable for transfer in infertility clinics. In vitro culture of preimplantation embryos has been associated with a number of perturbations, including ultrastructure, gene expression, metabolism and post-transfer development. We report here the transcriptional profiles characteristic of ESC lines generated from either in vitro cultured or in vivo derived embryos.

8

Harvey AJ, Mao S, Lalancette C, Krawetz SA, Brenner CA