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Accession IconGSE12882

Replacing skeletal muscle alpha-actin with cardiac actin in mouse skeletal muscle

Organism Icon Mus musculus
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina mouse-6 v1.1 expression beadchip

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Skeletal muscle actin mice (Crawford et al., (2002) Mol Cell Biol 22, 5587) were crossed with cardiac actin transgenic mice (termed "ACTC^Coco" or "Coco" for short), to produce mice that had cardiac actin instead of skeletal muscle actin in their skeletal muscles (termed "ACTC^Co/KO" or for short "Coco/KO"). Microarray analysis using the Illumina mouse-6 v1.1 expression beadchip was performed on RNA extraced from the soleus muscle of Coco/KO mice and wildtype mice, to confirm the swith in actin isoform expression, and to determine what other differences might exist between wildtype mice and the Coco/KO mice.
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