Our previous study has showed Ten-eleven translocation (Tet2) gene expression as well as the global 5-hydroxymethylcytosine (5hmC) level were reduced significantly in mouse kidney insulted by ischemia reperfusion (IR). Here, we investigate the functional role of Tet2 in renal IR injury. We constructed renal IR injury model in wild type and Tet2 knockout (KO) mice. Serum creatinine (SCr) and blood urea nitrogen (BUN) were examined to evaluate renal function. HE staining and renal injury biomarkers were tested to confirm the degree of injury. Microarray analysis of mRNA expression levels was carried out to gain mechanistic insightinto the role of Tet2.