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Accession IconGSE116164

Deficiency in SUMOylation, by injection of Gam1 mRNA into one cell embryos, leads to differential expression of genes in Xenopus

Organism Icon Xenopus laevis
Sample Icon 18 Downloadable Samples
Technology Badge Icon Affymetrix Xenopus laevis Genome 2.0 Array (xlaevis2)

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Background: Adenovirus protein Gam1 triggers the proteolytic destruction of the E1 SUMO-activating enzyme. Microinjection of an empirically determined amount of Gam1 mRNA into one-cell Xenopus embryos can reduce SUMOylation activity to undetectable, but nonlethal levels, enabling an examination of the role of this post-translational modification during early vertebrate development.
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