Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3.

To investigate the importance of STAT3 in the establishment of ES cells we have in a first step derived stable pluripotent embryonic stem cells from transgenic FVB mice expressing a conditional tamoxifen dependent STAT3-MER fusion protein. In a second step, STAT3-MER overexpressing cells were used to identify STAT3 pathway-related genes by expression profiling in order to identify new key-players involved in maintenance of pluripotency in ES cells.

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Cinelli P, Casanova EA, Uhlig S, Lochmatter P, Matsuda T, Yokota T, Rülicke T, Ledermann B, Bürki K