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Accession IconGSE107537

The effect of simulated night shift work on the circadian regulation of the human transcriptome

Organism Icon Homo sapiens
Sample Icon 100 Downloadable Samples
Technology Badge Icon Affymetrix Clariom S Pico Assay HT (clariomshumanht)

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Eight healthy human subjects were enrolled in a 6-day simulated shift work protocol. Blood samples were collected during the two 24-hour measurement periods. Blood samples were collected every 4 hours during both measurement periods. Subjects entered the lab on Day 1. At the start of Day 2, the first 24-hour measurement period was started. Subjects slept according to their habitual sleep/wake schedule, followed by a 16-hour constant posture procedure. On days 3-6, the sleep period was delayed by 10 hours. Following the third night on this schedule, subjects underwent another 24-hour measurement period. During both measurement periods, 7 blood samples were collected and PBMCs were isolated. mRNA was extracted, labelled, and hybridized to microarrays.
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