Transcriptomic profiling of antigen-specific T cells at the single-cell level after clearance of hepatitis C virus

We used scRNA-seq data generated using Smart-seq2 to reconstruct the native TCRαβ from Ag-specific T cells and then to link these with the gene expression profile of individual cells. antigen specific CD8 T cells were obtained from a subject who had previously cleared hepatitis C virus (HCV) infection. A cell line was also generated from the same subject and included in the analysis with and without antigen stimulation.

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