Illumina sequencing of library 16564904, constructed from sample accession ERS1079138 for study accession ERP014517.  This is part of an Illumina multiplexed sequencing run (19850_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

RNA from a pool of 12 single wild type zebrafish embryos plus ERCC spike mix 2 (Ambion). The sample has been DNAse treated.

ZMP_phenotype_115_B

RNA Seq data was generated from RNA of wild type zebrafish embryo pools at different stages of development for baseline transcriptional profiling

Establish baseline expression transcriptional signatures for zebrafish developmental stages. Total RNA was extracted from single wild type zebrafish embryos at a series of different developmental stages. RNA from 12 embryos was pooled to generate a replicate.The RNA was DNase treated.  Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Baseline_expression_from_transcriptional_profiling_of_zebrafish_developmental_stages_3

Submitter Supplied Information

Samples