Illumina sequencing of library 15658885, constructed from sample accession ERS1023414 for study accession ERP013837.  This is part of an Illumina multiplexed sequencing run (18817_1).  This submission includes reads tagged with the sequence GCAACATT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 240 clutch 1 plus ERCC spike mix 2 (Ambion). A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph240

ZMP_phenotype_240_1_phenotypic_1

Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of zebrafish embryos over expressing prl3 by use of outcrossed tg(actin:prl3) fish comparing prl3 overexpression to wild type siblings

Over expression of prl3 by use of outcrossed tg(actin:prl3) fish comparing prl3 overexpression to wild type siblings.  The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Overexpression_of_prl3_in_zebrafish_embryos

Submitter Supplied Information

Samples