Illumina sequencing of library 15658791, constructed from sample accession ERS1022135 for study accession ERP013835.  This is part of an Illumina multiplexed sequencing run (18818_1).  This submission includes reads tagged with the sequence GCAACATT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single genotyped zebrafish embryo homozygous for hesx1, allele u768 plus ERCC spike mix 2. A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT.

hesx1_homozygous_A1

Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of zebrafish hesx1 embryos at 80% epiboly for transcriptional profiling

otal RNA was extracted from zebrafish embryos at 80% epiboly and genotyped for hesx1 to identify wild type, heterozygous and homozygous knockout embryos. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_hesx1_knockout_embryos

Submitter Supplied Information

Samples