Illumina sequencing of library 15414178, constructed from sample accession ERS991512 for study accession ERP013498.  This is part of an Illumina multiplexed sequencing run (18478_1).  This submission includes reads tagged with the sequence ATCACG.

Illumina HiSeq 2500 paired end sequencing

GBM_RNA6265221

Human glioblastoma stem cells are thought to be extremely important in causing recurrence of this aggressive brain tumour and resistance to therapy. However, little is known of the genes needed for these cells to survive. We are performing a genome-wide CRISPR/cas9 screen for the genes essential for proliferation of these cells in vitro. Such genes will have depletion of their guide RNAs, and these candidates will be validated in downstream in vitro and in vivo experimental work, representing potential novel therapeutic targets.

This is an in vitro genome-wide CRISPR/cas9 screen in human glioblastoma stem cells, screening for genes essential for survival of these cells. These cells express cas9 and have been transfected with a guide RNA library causing gene knockouts. We will analyse the sequencing data for depletion of guide RNAs. In this particular study, we will do RNA sequencing to correlate CRISPR with expression levels in specific cancer cell subpopulations. 
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

CRISPR_Screening_in_Glioblastoma

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