Zebrafish_eye_populations_transcriptomics

To characterise the transcriptome of the stem cells and the proliferative progenitors in the retina of 5-day old zebrafish (Danio rerio), eyes were prepared as follows: 20-30 eyes per condition were collected, dissociated and FACS sorted. The samples were collected to RLT buffer and RNA extraction was immediately performed. cDNA and Nextera XT libraries were generated, and subsequent sequencing was carried out using the Illumina HiSeq 2500 platform.

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