Illumina sequencing of library 15249482, constructed from sample accession ERS954832 for study accession ERP012920.  This is part of an Illumina multiplexed sequencing run (18222_1).  This submission includes reads tagged with the sequence GGACTCCTTAGATCGC.

Illumina HiSeq 2500 paired end sequencing

eye cell populations from zebrafish embryo

Danio rerio

To characterise the transcriptome of the stem cells and the proliferative progenitors in the retina of 5-day old zebrafish (Danio rerio), eyes were prepared as follows: 20-30 eyes per condition were collected, dissociated and FACS sorted. The samples were collected to RLT buffer and RNA extraction was immediately performed. cDNA and Nextera XT libraries were generated, and subsequent sequencing was carried out using the Illumina HiSeq 2500 platform.

The zebrafish eye develops from a neuroepithelial layer of retinal progenitors, which then become postmitotic and differentiate into the mature retinal cell types. After 72hpf, the embryonic development of the retina is completed, since all major cell types are present and arranged in the characteristic lamination of the vertebrate retina. At this point, the cells located in the central retina have already exited the cell cycle and almost all growth comes from a
stem cell niche located at the periphery of the retina, called the ciliary marginal zone (CMZ). The CMZ is an heterogeneous region composed of stem cells, which continuously supply the CMZ with intermediate progenitors, that gradually proliferate and differentiate moving towards the central retina, a process that spatially recapitulates the temporal progression of embryonic development. The aim of this project is to characterise the transcriptome of the stem cells and the proliferative progenitors in order to build gene networks within each compartment, and subsequently characterise the mechanisms by which a stem cell gives rise to a differentiated cell in a post-embryonic retina

     

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/