Illumina sequencing of library 15127999, constructed from sample accession ERS849288 for study accession ERP012128.  This is part of an Illumina multiplexed sequencing run (18147_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

Total RNA from a pool of 5 zebrafish embryos from the AB_Sanger strain at 54hpf plus ERCC spike mix 2 (Ambion).

Danio rerio

Zebrafish embryos were challenged with a range of pathogens and their mRNA expression profiles compared to mock challenged siblings using whole embryo RNA-seq

Total RNA was extracted from zebrafish embryos at different time points post pathogen infection. The RNA was DNase treated.  Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.

     

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

RNASeq_of_zebrafish_embryos_infected_with_pathogens

Submitter Supplied Information

Samples