Illumina sequencing of library 14913476, constructed from sample accession ERS847701 for study accession ERP012004.  This is part of an Illumina multiplexed sequencing run (17732_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single wild type zebrafish embryo from from the SAT (Sanger AB Tubingen) strain collected at 5dpf plus ERCC spike mix 2 (Ambion).  A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph207

Danio rerio

Paired-end sequence data from the IlluminaHiSeq was generated from zebrafish embryos from the SAT (Sanger AB Tubingen) strain for transcriptomic profiling

Total RNA was extracted from zebrafish embryos from the SAT (Sanger AB Tubingen) strain. The RNA was DNase treated.  The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. 

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_embryos_from_the_SAT__Sanger_AB_Tubingen__strain_2

Submitter Supplied Information

Samples