Illumina sequencing of library 13998972, constructed from sample accession ERS1138594 for study accession ERP010589.  This is part of an Illumina multiplexed sequencing run (16460_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single genotyped homozygous mutant embryo for ercc3, allele fh158, mock infected with 2% phenol red at 48 hpf from ZMP phenotype 189 clutch 1 plus ERCC spike mix 1 (Ambion).  A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph189

ZMP_phenotype_189_1_1

Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of mutant and wild type zebrafish larvae to investigate the molecular phenotypes associated with altered susceptibility to intracellular pathogen infection.

Total RNA was extracted from zebrafish embryos collected for one or more alleles of genes which mediate susceptibility to intracellular pathogens. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_mutants_infected_with_pathogens

Submitter Supplied Information

Samples