Illumina sequencing of library 13352614, constructed from sample accession ERS685787 for study accession ERP009909.  This is part of an Illumina multiplexed sequencing run (15851_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single phenotypically abnormal embryo obtained by crossing two fish heterozygous for dtb, allele 66ATUG plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT.

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared from RNA of mutant and wild type zebrafish biliary mutants for transcriptome profiling.

Total RNA was extracted from morpholigcally abnormal and wildtype sibling zebrafish biliary mutants initially identified in a chemical mutagenesis screen. The 3' ends of fragmented RNA were pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_biliary_mutants

Submitter Supplied Information

Samples