Illumina sequencing of library 13276519, constructed from sample accession ERS684551 for study accession ERP009868.  This is part of an Illumina multiplexed sequencing run (15788_1).  This submission includes reads tagged with the sequence TGCGATCT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from a single genotyped homozygous mutant embryo for dmd, allele ta222a, from ZMP phenotype 188 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph188

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared from RNA of mutant and wild type zebrafish larvae of muscle mutants for transcriptome profiling.

Total RNA was extracted from mutant and wildtype sibling larvae of one or more alleles of zebrafish muscle mutants. The 3' ends of fragmented RNA were pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_muscle_mutants_

Submitter Supplied Information

Samples