Illumina sequencing of library 10517277, constructed from sample accession ERS463304 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run (13256_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT.

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.

Total RNA was extracted from zebrafish embryos at a range of developmental stages which were treated with small molecules at different concentrations and from their corresponding solvent treated and untreated controls. The 3' end of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling

Submitter Supplied Information

Samples