Illumina sequencing of library 11904907, constructed from sample accession ERS554132 for study accession ERP007082.  This is part of an Illumina multiplexed sequencing run (14260_1).  This submission includes reads tagged with the sequence ATCACG.

Illumina HiSeq 2500 paired end sequencing

mRNA from a single 5 dpf genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1 plus ERCC spike mix 1 (Ambion).

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared for zebrafish embryos collected for one or more alleles identified by the Zebrafish Mutation Project for transcriptome profiling.

Total RNA was extracted from zebrafish embryos collected for one or more alleles identified by the Zebrafish Mutation Project (http://www.sanger.ac.uk/Projects/D_rerio/zmp/). The mRNA was pulled down using polyT oligos attached to magnetic beads and Illumina libraries were prepared using TruSeq Stranded mRNA SamplePreparation Kits (RS-122-2101 and RS-122-2102) according to manufacturer's instructions. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_mutants_from_the_zebrafish_mutation_project

Submitter Supplied Information

Samples