Illumina sequencing of library 9894426, constructed from sample accession ERS430947 for study accession ERP005517.  This is part of an Illumina multiplexed sequencing run (12655_1).  This submission includes reads tagged with the sequence TGCTGATA.

Illumina HiSeq 2500 paired end sequencing

3' end enriched mRNA from single morphologically abnormal zebrafish embryo from mitfa, allele w2 clutch 1 plus ERCC spike mix 2 (Ambion). A 8 base indexing sequence (TGCTGATA) is bases 11 to 18 of non-index read 2 followed by CG and polyT.

Danio rerio

Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of mutant and wild type zebrafish larvae of neural crest mutants for transcriptome profiling.

Total RNA was extracted from morphologically abnormal and wildtype sibling larvae of neural crest mutants derived from ENU mutagenesis. Total RNA was extracted from zebrafish embryos and DNase treated. Fragmented RNA was enriched for the 3’ ends by pulled down using a 30T polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 1 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 2 at the 5’ end followed by 10 bases HBDVHBDVHB, then one of 96 eight base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_neural_crest_mutants

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Samples