Illumina sequencing of library 9450580, constructed from sample accession ERS401951 for study accession ERP004581.  This is part of an Illumina multiplexed sequencing run (12207_1).  This submission includes reads tagged with the sequence TGCGATCT.

Illumina HiSeq 2000 paired end sequencing

3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGCGATCT)  is bases 13 to 20 of read 1 followed by CG and polyT.  More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared from mutant and wild type zebrafish embryos from the Zebrafish Mutation Project for transcriptome profiling

Total RNA was extracted from morpholically abnormal and sibling wild type embryos identified by the Zebrafish Mutation Project (http://www.sanger.ac.uk/Projects/D_rerio/zmp/). The 3’ end of fragmented RNA was pulled down using polyToligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using Illumina HiSeq paired-end sequencing. Protocol: Total RNA was extracted from zebrafish embryos using Trizol and DNase treated. Chemically fragmented RNA was enriched for the 3’ ends by pulled down using an anchored polyToligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_mutants_from_the_zebrafish_genome_project

Submitter Supplied Information

Samples