Illumina sequencing of library 9199357, constructed from sample accession ERS392479 for study accession ERP004579.  This is part of an Illumina multiplexed sequencing run (12002_1).  This submission includes reads tagged with the sequence ATCACGTT.

Illumina HiSeq 2000 paired end sequencing

3' end enriched mRNA from a single morphologically abnormal embryo from sox10, allele t3 clutch 1. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by polyT.

Danio rerio

Paired-end sequence data from the Illumina HiSeq was prepared from RNA of mutant and wild type zebrafish larvae of neural crest mutants for transcriptome profiling.

Total RNA was extracted from morpholigcally abnormal and wildtype sibling larvae of neural crest mutants derived from ENU mutagenesis. The 3’ end of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using Illumina HiSeq paired-end sequencing. Protocol: Total RNA was extracted from zebrafish embryos using Trizol and DNase treated. Chemically fragmented RNA was enriched for the 3’ ends by pulled down using an anchored polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_zebrafish_neural_crest_mutants

Submitter Supplied Information

Samples