Illumina sequencing of library 6502565, constructed from sample accession ERS204891 for study accession ERP002113.  This is part of an Illumina multiplexed sequencing run (9221_8).  This submission includes reads tagged with the sequence ATCACG.

Illumina HiSeq 2000 paired end sequencing

Worm heads were amputated and exposed to PZQ 90uM for 1hr. Media was removed, samples homogenized in trizol (1.5ml)

Dugesia japonica

Praziquantel mode of action and resistance

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

The drug Praziquantel is the most commonly used drug for parasitic flatworms. It is currently being increasingly used in mass drug administration programmes, raising concerns over whether resistance will develop. Although widely used, its mode of action was until very recently uncertain.  This study will investigate the praziquantel mode of action and resistance by sequencing the transcriptomes of Dugesia japonica and different stages and strains of S. mansoni.

Praziquantel mode of action and resistance

Submitter Supplied Information

Samples