Illumina sequencing of library 6469770, constructed from sample accession ERS195616 for study accession ERP001991.  This is part of an Illumina multiplexed sequencing run (9201_6).  This submission includes reads tagged with the sequence ATCACG.

Illumina HiSeq 2000 paired end sequencing

Morphologically abnormal zebrafish embryos from zmp_ph 7 knockout incross 1.  More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph7

Danio rerio

Paired-end sequence data from the IlluminaHiSeq was prepared from mutant and wild type zebrafish embryos for transcriptome profiling

The transcriptome of zebrafish with morphologically mutant phenotypes associated with knockout alleles were analyised using Illumina sequencing to detect differential transcript abundance.  Total RNA was extracted from morpholically abnormal and sibling wild typeembryos from 3 clutches using Trizol.  After DNase treatment non-directional RNAseq libraries were made using the TrueSeq protocol with sample indexing.  Libraries were 75 base paired-end sequenced by Illumina HiSeq. 
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

RNAseq_vs_DeTCT_comparison

Submitter Supplied Information

Samples