Illumina sequencing of library 5665262, constructed from sample accession ERS159417 for study accession ERP001656.  This is part of an Illumina multiplexed sequencing run (8295_6).  This submission includes reads tagged with the sequence GAGGC.

Illumina HiSeq 2000 paired end sequencing

3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype1clutch1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT.  More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph1

Danio rerio

Paired-end sequence data from the IlluminaHiSeq was prepared from mutant and wild type zebrafish embryos from the Zebrafish Mutation Project for transcriptome profiling

Total RNA was extracted from morpholically abnormal and sibling wild type embryos identified by the Zebrafish Mutation Project (http://www.sanger.ac.uk/Projects/D_rerio/zmp/). The 3’ end of fragmented RNA was pulled down using polyToligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted from mouse embryos using Trizol and DNase treated. Chemically fragmented RNA was enriched for the 3’ ends by pulled down using an anchored polyToligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyToligo with part of Illumina adapter 1 at the 5’ end followed by 4 random bases, then an A, C or G base, then one of twelve5 base indexing tags and 14 T bases. An Illumina library with full adapter sequence was produced by 15 cycles of PCR. 

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Transcriptome_profiling_of_mutants_from_the_zebrafish_genome_project

Submitter Supplied Information

Samples