Sanger Institute S. pombe array 3.1.1 template 6.3

Sanger Institute S. pombe array 3.1.1 template 5.5

Sanger Institute S. pombe array 3.1.1 template 5.4

Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]

The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.

The Fission Yeast Homeodomain Protein Yox1p Binds to MBF and Confines MBF-dependent Cell-Cycle Transcription to G1-S via Negative Feedback

rawData

scan

processedDataMatrix

Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).

Genotype

StrainOrLine

time

time(min)

Transcription profiling of fission yeast wild type and yox1 mutant strains

Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).


Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-2315

P-MEXP-2316

P-MEXP-2317

P-MEXP-2318

P-MEXP-2319

P-MEXP-3086

P-TABM-1328

Cells were exponentially grown in Edinburgh minimal medium (EMM; Moreno et al., 1991,  Methods Enzymol. 194, 795-823.) at 32C to a titer under 5 x 10E6 cells/ml.

P-TABM-1329

P-TABM-3614

5L of yox1::kanMX6 ade6-M? h+ cells were grown at 30°C to OD600 ~0.2, before loading into a Beckman J6 centrifuge with JE-5.0 elutriation rotor and elution at 30°C with 80% fresh EMM, 20% conditioned EMM, 0.005% YE. Samples were collected every 15 min for ~2 h. A 150 mL sample of exponentially growing cells was collected before loading into the centrifuge and used as reference.

Transcription profiling of fission yeast wild type and yox1 mutant strains

Submitter Supplied Information

Samples