Affymetrix GeneChip Human Genome HG-U133A [HG-U133A]

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Comparison of gene expression of cancerous and non cancerous lung adenocarcinoma tissue. Tumour and normal samples from 18 patients plus tumour only from 5 patients.

disease staging

Transcription profiling of cancerous and non cancerous lung adenocarcinoma tissue. Tumour and normal samples from human lung carcinoma from 18 patients plus tumour only from 5 patients

Comparison of gene expression of cancerous and non cancerous lung adenocarcinoma tissue. Tumour and normal samples from 18 patients plus tumour only from 5 patients.


Affymetrix:Protocol:Hybridization-EukGE-WS2v4

Title: Fluidics Station Protocol. Description:

Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

Total RNA was isolated from human lung cancer tumor tissue and adjacent non cancerous lung tissue using RNeasy midi kit (Qiagen sciences Maryland USA)

cDNA was synthesized from 8 ug Total RNA using Superscript double stranded cDNA synthesis kit (Invitrogen CA USA).Biotynilated cRNA was then synthesized using the ENZO bioarray high yield RNA transcript labeling kit (Enzo life sciences Inc., Farmingdale NY USA). Labeled probe was purified by RNeasy mini kit columns according to manufacturer's protocol. (Qiagen sciences Maryland USA).

Transcription profiling of cancerous and non cancerous lung adenocarcinoma tissue. Tumour and normal samples from human lung carcinoma from 18 patients plus tumour only from 5 patients

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