Affymetrix GeneChip Bovine Genome Array Bovine

Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus.

rawData

We examined gene expression induced by Rhipicephalus microplus bites on host skin of tick-resistant and tick-susceptible breeds of bovines, Nelore and Holstein respectively, when they underwent a primary infestation.

individual

phenotype

response to infestation

sampling time point

tick attachment to sample

Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus

Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus.

We examined gene expression induced by Rhipicephalus microplus bites on host skin of tick-resistant and tick-susceptible breeds of bovines, Nelore and Holstein respectively, when they underwent a primary infestation.


Total RNA was used to synthesize double-stranded cDNA using the One-Cycle cDNA Synthesis Kit. The cDNA served as a template to synthesize biotin-labeled antisense cRNA using an IVT Labeling Kit.

Chip hybridization, washing, and staining were performed according to the Affymetrix recommended protocols.

Signal intensities of each gene were obtained using the robust multiarray average (RMA).

The skin samples were collected with sterile biopsy punch from approximately six-months old calves.

For isolation of total RNA, skin biopsies were placed in RNALater solution  and stored at -80C until processing. Skin biopsies were frozen in liquid nitrogen and pulverized with a hammer. Trizon reagent in combination with SV Total RNA Isolation System kit were used to isolate total RNA from pulverized sample .

Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus

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