Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]

Construction of regulatory networks using expression time-series data of a genotyped population

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We profiled the expression levels of three single deletion mutants (ARO80, DAT1, RTG3) of the the wild type strain BY4742 50 minutes after rapamycin perturbation.  Our goal is to validate regulatory networks inferred from microarray data E-MTAB-412.

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Transcription profiling by array of yeast ARO80, DAT1 and RTG3 deletion mutants after rapamycin perturbation

Construction of regulatory networks using time series genotyped microarray data

We profiled the expression levels of three single deletion mutants (ARO80, DAT1, RTG3) of the the wild type strain BY4742 50 minutes after rapamycin perturbation.  Our goal is to validate regulatory networks inferred from microarray data E-MTAB-412.


P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MTAB-17771

A 70 ml YPD culture of each parental strain and segregant was grown to log phase in shaken flasks at 30¡C.

P-MTAB-17772

Rapamycin was added to the culture at a concentration of 100ÊnM to induce perturbations in gene expression.

P-MTAB-17773

Total RNA was prepared from these cell samples using RNeasy kits from Qiagen

P-MTAB-17774

P-MTAB-17775

SCANNING

Arrays were scanned using an Affymetrix GeneChip 3000 array scanner.

P-MTAB-17777

Filter out S. pombe probe sets. Summarized expression values using the expresso function in the affy Bioconductor package, using rma as the background correction method, quantile array normalization, and median polish to summarize probe intensity data. Standard RMA transformation

Transcription profiling by array of yeast ARO80, DAT1 and RTG3 deletion mutants after rapamycin perturbation

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