Affymetrix GeneChip E.coli Genome 2.0 Array [E_coli_2]

Physiological response of Escherichia coli O157:H7 Sakai to dynamic changes in temperature and water activity as experienced during carcass chilling

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processedData

This experiment contains the transcriptomic dataset that constitutes part of an integrated transcriptomic and proteomic study monitoring the response of exponential phase E. coli O157:H7 Sakai cultures upon an abrupt downshift in temperature and water activity (from 35°C aw 0.993 to 14°C aw 0.967).

growth condition

sampling time

Transcription profiling by array of Escherichia coli O157:H7 Sakai subjected to an abrupt downshift in temperature and water activity at multiple time points

This experiment contains the transcriptomic dataset that constitutes part of an integrated transcriptomic and proteomic study monitoring the response of exponential phase E. coli O157:H7 Sakai cultures upon an abrupt downshift in temperature and water activity (from 35ÃÂ°C aw 0.993 to 14ÃÂ°C aw 0.967).

Molecular response of Escherichia coli O157:H7 Sakai during dynamic changes in growth kinetics induced by an abrupt downshift in temperature and water activity

This experiment contains the transcriptomic dataset that constitutes part of an integrated transcriptomic and proteomic study monitoring the response of exponential phase E. coli O157:H7 Sakai cultures upon an abrupt downshift in temperature and water activity (from 35°C aw 0.993 to 14°C aw 0.967).


P-MTAB-35566

Affymetrix:Protocol:Hybridization-Unknown

For the control samples the 'secondary' culture was harvested once it reached the mid-exponential growth phase (OD600 of 0.1 ± 0.01). These cultures were not subjected to any treatments.

Experimental samples were grown to mid-exponential growth phase (OD600 of 0.1 ± 0.01) and then subjected to a sudden downshift in both temperature and water activity, by adding an appropriate volume of NaCl-concentrated BHI to yield a final aw of 0.967 and by placing the culture at 14°C in a shaking water bath. Samples were harvested at 60, 250, 1605, 4070, 5700, 9900 and 18565 minutes after the downshift.

Transcription profiling by array of Escherichia coli O157:H7 Sakai subjected to an abrupt downshift in temperature and water activity at multiple time points

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