Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]

The histone demethylase activity of Rph1 is not essential for its role in the transcriptional response to nutrient signaling.

rawData

Gene regulatory significance of the JmjC domain H235A point mutation in the yeast transcription factor Rph1, in log and post-diauxic shift (pds) phase

genotype

growth condition

Transcription profiling by array of wild type, gis1 knockout, rph1 knockout, gis1rph1 double knockout, rph1-H235A mutant and gis1 knockout/rph1-H235A mutant yeast at different growth phases

Transcription profiling of wild type, gis1 knockout, rph1 knockout, gis1rph1 double knockout, rph1-H235A mutant and gis1 knockout/rph1-H235A mutant yeast at different growth phases

The histone demethylase activity of Rph1 affects telomeric silencing but is not required for nutrient signaling

Gene regulatory significance of the JmjC domain H235A point mutation in the yeast transcription factor Rph1, in log and post-diauxic shift (pds) phase


P-AFFY-3

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MTAB-36502

Yeast cultures were grown in rich YPD media. Overnight pre-cultures were diluted to an OD600 of 0.1, and then kept in continuous log phase by repeated dilutions during 24 hours to ensure that no stationary phase transcripts remained. After the final dilution to an OD600 of 0.1, log phase cells were harvested after 3 h of growth (at an OD600 of 0.4) and pds phase cells were harvested after 12 h of growth (at an OD600 of 7). Harvest was done by pelleting the cells by centrifugation for 5 min, followed by immediate freezing in liquid nitrogen. 

P-MTAB-36503

RNA was prepared from frozen yeast cells using the RiboPure Yeast kit (Ambion). 

Transcription profiling by array of wild type, gis1 knockout, rph1 knockout, gis1rph1 double knockout, rph1-H235A mutant and gis1 knockout/rph1-H235A mutant yeast at different growth phases

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