The effects of constitutively active Hypoxia Inducible Factor (HIF) and inactivated von Hippel-Lindau tumor suppressor gene product (pVHL) were examined in a mouse model.  Conditionally expressed, constitutively active HIF-1a and HIF-2a were compared with inactivated pVHL.

Transcription profiling of liver from mice with constitutively active Hypoxia Inducible Factor (HIF-1a and HIF-2a) or inactivated von Hippel-Lindau tumor suppressor gene product (pVHL)

Failure to prolyl hydroxylate hypoxia-inducible factor alpha phenocopies VHL inactivation in vivo

Affymetrix GeneChip Mouse Genome 430A 2.0 [Mouse430A_2]

rawData

scan

genotype

Transcription profiling by array of mice with liver-specific expression of hypoxia inducible factor, or inactivated von Hippel-Lindau tumor suppressor

The effects of constitutively active Hypoxia Inducible Factor (HIF) and inactivated von Hippel-Lindau tumor suppressor gene product (pVHL) were examined in a mouse model.  Conditionally expressed, constitutively active HIF-1a and HIF-2a were compared with inactivated pVHL.


Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-28883

Samples purified using Trizol.  Amplification and labelling was performed using in vitro transcription per Affymetrix protocols.

P-MEXP-31998

P-MEXP-32850

Mouse Colony Generation and Maintenance The albumin-Cre (Alb-Cre) C57/B6 mouse strain was purchased from Jackson Labs (Bar Harbor, Maine). The VHL f/f Balb/C mouse a generous gift from Volker Haase (University of Pennsylvania).  Genomic DNA was isolated from mouse tails using a Puregene DNA Isolation Kit (Gentra Systems) as per manufacturer’s instructions.  Mice were genotyped by PCR (primers and conditions available upon request). All procedures involving mice were performed in accordance with the National Institutes of Health guidelines for the use and care of live animals and were approved by the Dana-Farber Cancer Institute Animal Care and Use Committee.

Transcription profiling by array of mice with liver-specific expression of hypoxia inducible factor, or inactivated von Hippel-Lindau tumor suppressor

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