Transcription profiling by array of Arabidopsis mutant for brx after treatment with brassinolide or indole-3-acetic acid

We compared the seedling transcription profiles to determine the effects of loss-of-function of the BRX gene of Arabidopsis. BRX is required for optimal root growth. We compared seedlings of a loss-of-function line (brx) with its control background (Sav-0). Because the loss-of-function line was derived from introgression, a brx line that was complemented by a transgenic wild type copy of BRX was also included as a control. This line (rescued brx) allows the identification of expression differences that are due to introgression drag. See Mouchel et al. 2004, Genes & Dev. Vol. 18, p. 700 for a detailed description. We also compared to response of the different genotypes to the application of the phytohormones brassinolide (BL) and indole acetic acid (IAA)

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Mouchel CF, Osmont KS, Hardtke CS