Gene expression profiling of the medial (MGE), lateral (LGE) and caudal (CGE) ganglionic eminence, and cerebral cortex (CTX) at various embryonic stages (E12.5, E14 and E16).

Transcription profiling of rat ganglionic eminences and cerebral cortex at embryonic stages E12.5, E14 and E16

Comprehensive spatiotemporal transcriptomic analyses of the ganglionic eminences demonstrate the uniqueness of its caudal subdivision

Affymetrix GeneChip Rat Expression Array RAE230A [RAE230A]

Affymetrix GeneChip Rat Genome 230 2.0 [Rat230_2]

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scan

processedDataMatrix

developmental stage

organism part

Gene expression profiling of the medial (MGE), lateral (LGE) and caudal (CGE) ganglionic eminence, and cerebral cortex (CTX) at various embryonic stages (E12.5, E14 and E16).


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450[]

Affymetrix:Protocol:Hybridization-EukGE-WS2v5_450[]

Affymetrix:Protocol:Hybridization-[]

Affymetrix:Protocol:MAS4:ExpressionCall

Title: Affymetrix CHP Analysis (ExpressionCall). Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-20176

Total RNA was isolated with the RNAqueous kit (Ambion), precipitated with 2.5 volumes of 95% EtOH and 0.1 volume of 3M sodium acetate (pH 5.2), 3x washed with 80% EtOH, and resuspended in distilled water. RNA quality was assessed with the 2100 Bioanalyzer (Agilent).

P-MEXP-38224

P-MEXP-38303

P-MEXP-52627

The cerebral cortices (CTX) of 10 embryos (1 litter) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual.

P-MEXP-52628

The CGEs of 10 embryos (1 litter) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual. 

P-MEXP-52629

The cerebral cortices (CTX) of 10 embryos (1 litter) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual. 

P-MEXP-52630

The LGEs of 10 embryos (1 litter) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual.

P-MEXP-52631

The MGEs of 10 embryos (1 litter) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual.

P-MEXP-52632

The cerebral cortices (CTX) of 30 embryos (2-3 litters) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual.

P-MEXP-52633

The MGEs of 30 embryos (2-3 litters) were lysed in 0.7ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto a RNAqueous Filter Cartridge for subsequent extraction of the total RNA according to the RNAqueous kit manual.

P-MEXP-52634

The CGEs of 70 embryos (6-7 litters) were lysed in 2.1ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto 3 RNAqueous Filter Cartridges for subsequent extraction of the total RNA according to the RNAqueous kit manual. At the final step, elutes of the three Filter Cartridges were pooled together. 

P-MEXP-52635

The LGEs of 70 embryos (6-7 litters) were lysed in 2.1ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto 3 RNAqueous Filter Cartridges for subsequent extraction of the total RNA according to the RNAqueous kit manual. At the final step, elutes of the three Filter Cartridges were pooled together. 

P-MEXP-52636

The MGEs of 70 embryos (6-7 litters) were lysed in 2.1ml Lysis Buffer (RNAqueous kit, Ambion), equal volume of 64% EtOH was added, and the lysate/ethanol mixture was applied onto 3 RNAqueous Filter Cartridges for subsequent extraction of the total RNA according to the RNAqueous kit manual. At the final step, elutes of the three Filter Cartridges were pooled together. 

Transcription profiling of rat ganglionic eminences and cerebral cortex at embryonic stages E12.5, E14 and E16

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