Transcription profiling of mouse otic vesicle and surrounding mesenchyme and neighboring hindbrain sample from wild type and mouse mutants for FGF3, FGF10 and FGF3/FGF10 double mutants at embryonic day E10

Wild-type and mouse mutants for FGF3, FGF10 and FGF3/FGF10 double mutants at embryonic day E10 were analysed by microarrays for downregulated genes. A tissue sample corresponding to an area containing the otic vesicle and surrounding mesenchyme and neighboring hindbrain were isolated from E10 embryos (See Figure 3A of manuscript). Five samples were pooled for RNA preparation. Samples were isolated from wild-type, FGF3, FGF10 and FGF3/FGF10 double mutants. Two RNA samples for each genotype were generated (corresponding to 8 tissue samples). RNA was labeled and hybridized with Affymetrix U74A V2 arrays.

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Lioubinski O, Alonso MT, Alvarez Y, Vendrell V, Garrosa M, Murphy P, Schimmang T