Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

rawData

processedData

The experiment was designed to enable comparison of ahk2 ahk3 double mutant in response to cold compared with wild types.

genotype

temperature

Transcription profiling by array of genes regulated by ahk2 ahk3 in response to cold in Arabidopsis thaliana

The experiment was designed to enable comparison of ahk2 ahk3 double mutant in response to cold compared with wild types.


Affymetrix:Protocol:Hybridization-Unknown

Affymetrix:Protocol:MAS4:ExpressionCall

Title: Affymetrix CHP Analysis (ExpressionCall). Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-MTAB-28026

Total RNA was isolated using RNeasy Mini Kit columns (Qiagen, Hilden, Germany). RNA quality was assessed by Agilent 2100 bioanalyser using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and quantity was determined by ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., DE, USA).(Parameters: Extracted product = total_RNA, Amplification = PCR)

P-MTAB-28027

Equal amounts of RNA from triplicate samples were pooled.

P-MTAB-28028

Arabidopsis thaliana was grown on germination agar plates containing 0.5x Murashige-Skoog (MS) media with vitamins, 1.5% sucrose, 2.5 mM Mes, pH 5.7, and 0.8% agar at 23C with a 16-h photoperiod for 11 days(Parameters: time unit = hours, temperature unit = C, media = 1/2 MS media)

P-MTAB-28029

Arabidopsis thaliana was grown on germination agar plates containing 0.5x Murashige-Skoog (MS) media with vitamins, 1.5% sucrose, 2.5 mM Mes, pH 5.7, and 0.8% agar at 23C with a 16-h photoperiod for 11 days. For cold treatment, the light-grown seedlings were incubated at 1C for 2 h with white fluorescent light. (Parameters: time unit = seconds, temperature unit = C, media = 1/2 MS media)

P-MTAB-28030

P-MTAB-28031

Affymetrix CEL analysis(Parameters: Scanning hardware = GeneChip Scanner 3000 [Affymetrix], Scanning software = MAS/GCOS/GREX [Affymetrix])

Transcription profiling by array of genes regulated by ahk2 ahk3 in response to cold in Arabidopsis thaliana

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Samples