Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

Effects of aneuploidy on genome structure, expression, and interphase organization in Arabidopsis thaliana

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Effects of aneuploidy on gene expression in Arabidopsis thaliana containing extra copies of chromosome 5.

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Transcription profiling of A.thaliana to determine the ffect of aneuplody extra copy of chromosome 5

Effects of aneuploidy on gene expression in Arabidopsis thaliana containing extra copies of chromosome 5.


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450

Title: Affymetrix EukGE-WS2v4_450 Hybridization. Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v5_450

(Parameters: Extracted product = total_RNA, Amplification = none)

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-134916

Seeds were germinated on sterile, solid Murashige and Skoog (MS) medium in plastic petri dishes. Seedlings were then transferred to pots containing a mixture of Huminsubstrat N3 and Vermiculit Nr.2 (2:1 v/v) (purchased from a local supplier), and placed in a culture room with natural light (3000 lux). The photoperiod was 16 h and temperature was maintained at 23 °C. Single leaves were cut from the plants at a stage of approximately ten rosette leaves (>1 cm in length).

P-MEXP-135114

Total RNA was extracted and purified with an RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA was quantified by spectrophotometry (Nanodrop ND-1000, Peqlab, Erlangen, Germany). Double-stranded cDNA was synthesized from 1 ug total RNA by using the one-cycle cDNA synthesis kit (GeneChip One-Cycle Target Labeling and Control Reagents, Affymetrix, High Wycombe, U.K.) 

Transcription profiling of A.thaliana to determine the ffect of aneuplody extra copy of chromosome 5

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