Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

MADS-complexes regulate transcriptome dynamics during pollen maturation

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processedDataMatrix

Effects of loss-of-function of AtMIKC* MADS-box genes on the mature Arabidopsis pollen transcriptome.

genotype

Transcription profiling of mature pollen grains from wild type and AtMIKC* MADS-box gene knock-out Arabidopsis plants

Effects of loss-of-function of AtMIKC* MADS-box genes on the mature Arabidopsis pollen transcriptome.


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-Unknown

Affymetrix:Protocol:MAS4:ExpressionCall

Title: Affymetrix CHP Analysis (ExpressionCall). Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-64246

Arabidopsis thaliana Col-0 plants were grown under standard greenhouse conditions, with temperature controlled at 22ºC and 16 hours of light at around 120 μmol m-2 s-1.

P-MEXP-64247

At a fixed time in the morning mature pollen grains were harvested from open flowers by shaking in 0.3 M mannitol, as described by Honys and Twell (Genome Biol, 2003). For total RNA isolation with the RNeasy kit (Qiagen) pollen was ground with quartz sand, and RNA was stored at -80°C.

P-MEXP-64259

Mature pollen grains were ground with quartz sand, and total RNA was isolated with the RNeasy kit (Qiagen).

Transcription profiling of mature pollen grains from wild type and AtMIKC* MADS-box gene knock-out Arabidopsis plants

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