The experiment describes the dynamic transcriptional alterations in brains of ME7- infected, and age-matched, mock-inoculated mice immediatly before inoculation, at two important preclinical time points and at terminal stages.

Transcription profiling of mouse scrapie ME7-infected and mock-infected brains at two important preclinical time points and at terminal stages

Transcriptome analysis reveals altered cholesterol metabolism during the neurodegeneration in mouse scrapie model

Affymetrix GeneChip Mouse Expression Array MOE430A [MOE430A]

rawData

scan

processedDataMatrix

infect

time

Transcription profiling by array of brains from mice infected with scrapie ME7

The experiment describes the dynamic transcriptional alterations in brains of ME7- infected, and age-matched, mock-inoculated mice immediatly before inoculation, at two important preclinical time points and at terminal stages.


Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450

Title: Affymetrix EukGE-WS2v4_450 Hybridization. Description:

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-43454

 All mice were housed under controlled lighting(12h light/darkness), temperature (21-22°C)and humidity(60-65%) under standard IVC (individual ventilated cages) barrier conditions in the same animal facility and allowed access to food and water ad libitum.   

P-MEXP-45687

The animal was sacrificed at the age of 42 days without any treatment.

P-MEXP-45688

42-day-old mouse was intracerebrally inoculated with 30µl of a 10% brain homogenate of healthy mice and killed at 90 dpi

P-MEXP-45689

42-day-old mouse was intracerebrally inoculated with 30µl of a 10% brain homogenate of healthy mice and killed at 120 dpi 

P-MEXP-45690

42-day-old mouse was intracerebrally inoculated with 30µl of a 10% brain homogenate of healthy mice and killed at 150 dpi 

P-MEXP-45691

42-day-old mouse was intracerebrally inoculated with 30 µl of a 10% brain homogenate of ME7-infected mice, and killed at 90 dpi.

P-MEXP-45692

42-day-old mouse was intracerebrally inoculated with 30 µl of a 10% brain homogenate of ME7-infected mice, and killed at 120 dpi

P-MEXP-45693

42-day-old mouse was intracerebrally inoculated with 30 µl of a 10% brain homogenate of ME7-infected mice, and killed upon display of terminal clinical signs associated with prion disease (around 150 dpi)

P-MEXP-45695

Total RNAs were prepared with a phenol-guanidine isothiocyanate reagent (Trizol; Invitrogen, Karlsruhe, Germany) and cleaned by the use of RNeasy columns (Qiagen, Hilden, Germany). From 21-µg samples of total RNA, first-strand cDNAs were synthesized with SuperScript II (Invitrogen) and the T7-(dT24) primer (MWG, Ebersberg, Germany), followed by the generation of double-stranded cDNAs. The resultant cDNAs were purified by use of a GeneChip sample clean-up module (Affymetrix) and served as templates for the generation of biotinylated antisense RNAs (cRNAs) by use of an EnzoBioarray kit (Affymetrix). The biotinylated cRNAs were hybridized to Mouse Expression Arrays 430A (ME430A; Affymetrix)

P-MEXP-9649

Normalization with RMA; all hybridisations merged to one expression set

Transcription profiling by array of brains from mice infected with scrapie ME7

Submitter Supplied Information

Samples