A. Experimental design: 1 genotype: Sachiyutaka. 2 treatments: thinning at R5 (plant population density was altered from 22.2 plants to 5.56 plants per square meter) and control (plant population density was maintain at 5.56 from sowing to harvest). 2 replications 2 time point: 14 days after R5 and 28 days after R5. TOTAL: 8 RNA sequencing. Sample: main stem. Stage: R5 to R6. Growth condition: Field condition. Day length: Field condition. Sampling time: 9 a.m. to 10 a.m. Day length: Field condition. Sampling time: 9 a.m. to 10 a.m.